Molecular characterization and chromosomal assignment of porcine BAX and TAC1
نویسندگان
چکیده
The BCL-2 associated X protein (BAX) is a member of the Bcl-2 protein family and functions as an accelerator of programmed cell death (apoptosis). The preprotachykinin A gene (TAC1) encodes two tachykinin peptides (substance P and neurokinin A) that act as neurotransmitters in the central and peripheral nervous systems. To isolate the genes from the porcine genomic PAC library TAIGP714 probes were generated with primers derived from exons of the human orthologs. For BAX, a 501-bp long fragment (spanning exons 3 to 4) was amplified on porcine genomic DNA (GenBank Accession no: AM233489). The TAC1-specific primers formed a 415-bp long amplicon (spanning exon 7) in pigs (GenBank Accession no: AM233488). Probe sequencing and comparison with the human genes verified sequence identity (BAX 94% and TAC1 84%). The gene-containing PAC clones were further isolated and sequenced. The chromosomal assignment of the genes was done by analyses of porcine hybrid panels (somatic cell and radiation hybrid panel) and by fluorescent in situ hybridization. BAX was assigned to SSC6q21 and TAC1 to SSC9q12-q14. So far, comparative exon sequencing using a panel of 138 animals (Angeln Saddleback, Pietrain, German Landrace, German Edelschwein, Swabian-Haellian swine, Buntes Bentheimer, Thai native pigs, Thai wild pig, Chinese Luchuan, Chinese Rongchang, Chinese Yushanei as well as German and Thai herniated crossbred piglets) identified two SNPs in BAX (SNPintron1: C→T, p(C)=0.804 and q(T)=0.196; SNPintron3: T→A, p(T)=0.975 and q(A)=0.025). Up to now, no SNPs have been found in TAC1. The chromosomal localization of BAX in combination with its known physiology proposes a possible contribution of the gene to the phenotype hernia, but this hypothesis has to be further elucidated.
منابع مشابه
Characterization of Avian Pasteurella multocida Isolates by Protein Profiles and Restriction Enzyme Analysis of Chromosomal DNA
متن کامل
Cytogenetic Analysis of Referral Cases with Growth Failure and Clinical Suspicion of having Chromosomal Abnormality
Background and Objective: Failure to thrive (FTT) is a sign that describes a particular problem rather than a diagnosis and explain growth failure or more advanced failure to gain weight appropriately. The aim of this study was to determine the prevalence and type of chromosomal abnormalities in patients presented with FTT. Materials and Method: One hundred FTT cases with clinical impressio...
متن کاملThe Effect of Dracocephalum kotschyi Alcoholic Extract on the BCL2 and BAX expression in SKBR3 Cell Line
Background: Breast cancer is the most prevalent malignancy among women in the worldwide and the outbreak in Iranian women is growing. Iranian patients with breast cancer are younger than their Western counterparts. Dracocephalum kotschyi is a wild-growing flowering plant belonging to the family Labiatae and found in Iran. This plant has been applied in Iran folk medicine. The aim of this study ...
متن کاملChanges of Genetic Loci among Gastric Cancer Patients, Southeastern Iran, 2017
Background: Characterization of genes and precise assessment of the number of copies are crucial for understanding the basis of emergence, progression, and identification of predictive markers of tumor malignancy. This study aimed to investigate the role of the changes in some central genes in gastric cancer. Materials and Methods: In this experimental study, 30 patients with gastric surgery w...
متن کاملAssociation of Fetal and Parental Chromosomal Abnormalities with Congenital Anomalies
Background & Aims: Chromosome abnormalities are a major cause of miscarriage and neonatal mortality. The present study aimed to determine the association of fetal and parents chromosomal abnormalities with congenital anomalies. Methods: A cross-sectional study was performed in a tertiary referral center (Afzalipour Hospital) over 16 months period (2011-2012). The study groups consisted of 77 fe...
متن کامل